The University of Calgary (UCalgary) Containment Level 3 (CL3) facility requires a single cell sequencing system to identify heterogeneous cells within complex tissues. The plan is to purchase the 10X Genomics Chromium X system, accessory kit with extended warranty. This highly versatile multiplex (multi-sample dependent on assay type), single-cell labelling system, allows for multiomic readouts of gene expression (scRNAseq), epigenetics (scATACseq), feature barcode technology to enable simultaneous profiling of additional cell surface/intracellular proteins and CRISPR profiling within a single cell.
The lab has also taken substantial amount of time to develop protocols on the current 10X Genomics Chromium systems which will be transferrable to the new system. The ability to interchange assays and data between Chromium systems is a mandatory requirement. Continuity is vital for the research to enable ongoing data comparisons and ensuring reliable research results.
